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$B!!(BThe rolling mouse Nagoya (tgrol/tgrol) is a spontaneous P/Q-type calcium channel mutant, which is suggested as an animal model for human neurologic disease such as autosomal dominant cerebellar ataxia (SCA6), familial hemiplegic migraine, and episodic ataxia type-2. Morphology of the spines is reported to reflect functional status of the neurons. To understand the functional status of Purkinje cell in calcium channel insufficiency, dendritic spines were analyzed quantitatively.
$B!!(BAfter rapid Golgi preparations of the rolling and wild cerebellum, the blocks were embedded in Epon-Araldite mixture. 4-mm sections were cut and observed with high voltage electron microscopy operated at 1,000 kV. Dendritic spine density and length of the spine were measured with NIH image.
$B!!(BMany spines emanating from the proximal dendrites in rolling mice were observed. The average spine density was 2.12 $B!^(B 0.18/10 mm in wild and 1.78 $B!^(B 0.22/10 mm in rolling. The average length of the spines was 1.12 $B!^(B 0.21 mm in wild and 0.82 $B!^(B 0.21 mm in rolling. The density and height of the spines were significantly decreased in rolling Purkinje cells (p $B!c(B 0.01).

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M. Ellisman, M. Martone, N. Yamada (Univ. Calif. NCMIR)

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A$B!K1QJ886CxO@J8(B

1): Noda T, Fujimoto K, Ide C (2001) Annulate lamellae are interconnected by three distinct cisternal structures.Acta Histochem. Cytochem. 34, 103-110.
2): Nagata, T (2001) Three-dimensional high voltage electron microscopy of thick biological specimens. Micron 32, 387-404.
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4): Kosaka K, Aika Y, Toida K, Kosaka T (2001) Structure of intraglomerular dendritic tufts of mitral cells and their contacts with olfactory nerve terminals and calbindin-immunoreactive type 2 periglomerular neurons. J. Comp. Neurol. 440, 219-235.
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B) $B3X2qH/I=(B

1): $BJR6ME8;R!$=E>>9/=(!$JR6M9/M:!J(B2001.2$B!KL5@TDGF0J*!JJ"B-N`!K$N30EeAH?%$KJ,I[$9$k6ZA!0]$N;0
2): $BF|9b!!fb!$5\CO1I0l!J(B2001.3$B!K?@7P7O$GH/8=$9$k%.%c%C%W7k9g%A%c%M%kCAGr!&%3%M%-%7%s$N2r@O!#Bh(B78$B2sF|K\@8M}3X2qBg2q!J5~ET!K(B
3): Toida K(2001.4) Cathecolaminergic Neurons in the Olfactory Bulb. The 9th Intern. Catecholamine Symposium (Joint Congress with "The 5th Intern. Conf. Progress in Alzheimer's and Parkinson's Disease") (Kyoto)
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5): $BJR6ME8;R!$=E>>9/=(!$M-0fC#IW!$JR6M9/M:!J(B2001.5$B!KJ"B-N`$N30EeAH?%$K$_$i$l$k6ZA!0]$ND69b05EE824Q;!$H$=$NN)BN:F9=C[!#F|K\EE;R82Hy6@3X2qBh(B57$B2s3X=Q9V1i2q!JJ!2,!K(B
6): $BHuED0lFA(B (2001.5) $B6&>GE@%l!<%6!<82Hy6@$rAH$_$"$o$;$?EE;R82Hy6@O"B3@ZJR:F9=C[K!$K$h$kSL5e%K%e!<%m%s9=@.$N;0
7): Hama K (2001.5) High voltage electron microscope in cell and tissue research. Korea Elect Micros Soc.(Korea)
8): $B>._70l;K!$Hi
9): $BJR6ME8;R!$=E>>9/=(!$M-0fC#IW!$JR6M9/M:(B(2001.10) $B%$%=%"%o%b%AMDF0J*$NHiIf8w3P:YK&$HFC0[$J@17A6Z:YK&$ND69b05EE824Q;!!#Bh(B72$B2sF|K\F0J*3X2qBg2q!JJ!2,!K(B
10): Toida K (2001.12) Neuronal organization in the rat olfactory bulb. The 6th Japan-China Joint Meeting on Histochemistry and Cytochemistry$B!JBh(B42$B2sF|K\AH?%:YK&2=3X2qAm2q!&3X=Q=82q7s:E!K(B($BEl5~(B)


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Copyright(C) 2002 National Institute for Physiological Sciences