トロポニン発見40周年記念国際シンポジウム

第33回生理研カンファレンス

Regulatory proteins of striated muscle
– structure, function and disorder–
〔筋収縮の調節タンパク質―構造，機能および疾患―〕
平成17年10月25日−10月28日
自然科学研究機構　岡崎コンファレンスセンター

　本シンポジウム(“Regulatory Proteins of Striated Muscle: Structure, Function and Disease”)は江橋節郎先生が筋収縮時のカルシウム調節タンパク質であるトロポニンを発見されてから40周年を迎えたことを記念して，大槻磐男（東京慈恵会医科大学客員教授）と岡田泰伸（生理学研究所細胞器官研究系教授）を組織委員長として平成17年10月25〜28日の4日間，岡崎コンファレンスセンターで開催された。
　海外から11名，国内から21名の筋収縮関連分野の代表的研究者による招待講演と国内外から31題のポスター発表が行われ，参加者延べ130人により4日間にわたって活発な議論が展開された。
　本シンポジウムの内容は，(1)トロポニンとトロポミオシンの制御，(2)心筋制御とその病態，(3)ミオシンの制御，(4)ECカップリングとその病態，(5)筋収縮タンパク質，(6)モーター分子などである。
　セッションの合間にA. F. Huxley卿とA. Weber教授からのトロポニン発見40周年のお祝いのメッセージが披露され，その返礼のスピーチが83才の江橋節郎先生から英語で行われ，参加者に深い感銘を与えた。

October 26
Opening remark　N. Mizuno (NIPS)
1.Regulation by troponin and tropomyosin　　（Chaired by I. Ohtsuki and Y. Maeda）

1.

I. Ohtsuki (Jikei Univ.)
Structure, Function and Disorder of Troponin

2.

J. Gergely (Boston Biomed, Res. Inst.)
Highlights of the regulation of striated muscle contraction by Ca²⁺

3.

Y. Maeda (RIKEN Harima)
Structural basis of troponin-tropomyosin mediated calcium regulation

4.

R. J. Fletterick (Univ. Calif.SanFrancisco)
Ca Ion and Troponin Switch

5.

B. J. Sykes (Univ. Alberta)
Structure and Dynamics of Calcium Regulatory Proteins ; from solution to intact muscle fibers

6.

T. Wakabayashi (Teikyo Univ.)
Structural basis for Ca²⁺-regulated muscle relaxation at interaction sites of troponin with actin and tropomyosin

7.

S. E. Hitchcock-DeGregori (Robert Wood Johnson Med Sch.)
Tropomyosin: A regulator of actin filament dynamics and contractile function

8.

L. S. Tobacman (Univ. Illinois)
The Thin Filament as a Regulatory Molecular Assembly

9.

M.A. Geeves (Univ. Kent)
Cooperativity in Tropomyosin and Troponin on the Thin Filament

10.

M. Miki (Fukui Univ.)
Conformational changes of the reconstituted skeletal muscle thin filament observed by fluorescence measurements

11.

T. Arata (Osaka Univ.)
Calcium structural transition of troponin in the complexes, on the thin filament, and in muscle fibers as studied by site-directed spin-labeling ESR.

12.

M. Tanokura (Univ. Tokyo)
Solution structure of the complex of troponin C with troponin I fragment from scallop striated adductor muscle

13.

Y. Nitanai (RIKEN, ERATO)
Crystal structure of tropomyosin: a flexible coiled-coil

14.

H. Kagawa ( Okayama Univ.)
A C. elegans model for studying troponin regulation of muscle contraction and animal behavior

October 27
2.Cardiac regulationand disorders　　(Chaired by R. J. Solaro and R.L. Moss)

15.

R. L. Moss (Univ. Wisconsin Med. Sch.)
Cooperative mechanisms in the activation of force and the kinetics of force development in striated muscles

16.

R. J. Solaro (Univ. Illiois Chicago, Center for Cardiovasc.Res.)
Heart Failure, Ischemia/Reperfusion Injury, and Cardiac Troponin

17.

J. Mogensen (Skejeby Univ. Hosp.)
Clinical troponin Imutationsin cardiomyopathies

18.

S. Morimoto (Kyushu Univ. Sch. Med. )
A knock-in mouse model for dilated cardiomyopathy caused by DK210 mutation in cardiac troponin T

19.

T.Toyo-oka (TUERO Touhoku Univ.)
Cardiac troponin as the most specific and sensitive biomarker for myocardial cell degradation in clinical practice

3.Regulation by myosin　　(Chaired by A. G. Szent-Gyorgyi and K. Kohama)

20.

A. G. Szent-Gyorgyi (Brandeis Univ.)
How calcium regulates some myosins: past and present

21.

K. Kohama (Gunma Univ.)
Calcium inhibition of Physarum myosin

4.EC-coupling and disorder　　(Chaired by Y. Ogawa and M. Iino)

22.

M. Endo (Saitama Med.Sch.)
Calcium-Induced Release of Calcium from the Sarcoplasmic Reticulum

23.

Y. Ogawa (Juntendo Univ. Med Sch)
Dysregulation of the gain of CICR through ryanodine receptor 1 (RyR1); the putative mechanism underlying malignent hyperthermia

24.

M. Iino (Univ. Tokyo)
Regulation of cell function by Ca²⁺ oscillation

25.

C. Toyoshima (Univ. Tokyo)
Ion pumping by cacium ATPase of sarcoplasmic reticulum

5.Contractile proteins　　(Chaired by K. Wakabayashi and N. Yagi)

26.

K. Wakabayashi (Osaka Univ.)
Roles of Structural Alterations of Thin Actin Filaments in Muscle Contraction

27.

H. E. Huxley (Brandeis Univ.)
The Length of the Active Crossbridge Stroke in Muscle during Steady Shortening

October 28
6.Motor proteins　　(Chaired by S. Ishiwata and T. Yanagida )

28.

S. Ishiwata (Waseda Univ.)
Cooperative Functions of Actomyosin Motors Focusing on the Auto-Oscillation (SPOC)

29.

T. Yanagida (Osaka Univ.)
A mechanism for the muscle contraction based on actin filament rotation

30.

T. Oda (RIKEN Harima)
Modeling of F-actin structure using electron microcopy and X-ray fiber diffraction

31.

K. Kinosita Jr (Waseda Univ.)
How Two-Foot Molecular Motors May Walk

32.

E. Katayama (Univ. Tokyo)
Actomyosin sliding as revealed by quick-freeze deep-etch replica electron microcopy of myosin crossbridges during in vitro motility assay

7.Closing session
Closing remarks