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14. The 39th NIPSI International Symposium

Frontiers of Biological Imaging
"Synergy of the Advanced Techniques"


November 10-12, 2008

National Institutes for Physiological Sciences
And
Okazaki Institute for Integrative Bioscience
Okazaki Conference Center, Okazaki, Japan
<http://www.nips.ac.jp/39symposium/ >


MONDAY, NOVEMBER 10
Opening address
(Kuniaki Nagayama, Okazaki Institute for Integrative Bioscience/National Institute for Physiological Sciences)
1. Tomomi Nemoto (National Institute for Physiological Sciences, Japan)
Potential of two-photon microscopy for analysis of living organ
2. Kira Poskanzer (Columbia University, USA)
Development of two-photon stimulation methods to map cortical circuits
3. Makio Tokunaga (National Institute of Genetics, Japan)
Highly inclined thin illumination enables clear single-molecule imaging in linving cells
4. Hiromi Okamoto (Institute for Molecular Science, Japan)
Potentiality of scanning near-field optical microscopy
5. Susy Kohout (University of California, USA)
Probing protein motions of Ci-VSP using voltage clamp fluorometry
6. Wolfgang Baumeister (Max-Planck-Institute, Germany)
Cryoelectron tomography: defining the functional modules of cells
7. Yasushi Hiraoka (Kobe Advanced ICT Research Center, Japan)
Correlative light and electron microscopy for observing molecular dynamics in living cells
8. John Sedat (University of California, USA)
New directions for live 4-dimensional imaging using OMX, a novel imaging platform
9. Masataka Murakami (National Institute for Physiological Sciences, Japan)
Salivary secretion: assessment of trans- and paracellular transport by physio-morphological techniques
10. Tomoko Nakanishi (The University of Tokyo, Japan)
Development of radioisotope imaging systems for plants
11. Haruo Sugi (Teikyo University, Japan)
Electron microscopic demonstration of the cross-bridge recovery stroke in living muscke thick filaments using the gas environmental chamber

TUESDAY, NOVEMBER 11
12. Yoshiyuki Kubota (National Institute for Physiological Sciences, Japan)
An excitatory and inhibitory synapse density on various GABAergic nonpyramidal cells in the rat cerebral cortex
13. CedricBouchet-Marquis (University of Colorado at Boulder, USA)
High resolution imaging using CEMOVIS and cryo-ET
14. Ohad Medalia (The Ben-Gurion University, Israel)
The molecular architecture of integrin-mediated focal adhesion by cryo-electron tomography
15. Wah Chiu (Baylor College of Medicine, USA)
Backbone tracing and model building in single particle cryo-EM
16. Holger Stark (Max-Planck-Institute, Germany)
Studying 3D dynamics of macromolecular machines by electron cryomicroscopy
17. Takashi Ishikawa (ETH Zurich, Switzerland)
Molecular arrangement of dynein in flagella revealed by cryo-electron tomography
18. Abraham Koster (Leiden University Medical Center, The Netherlands)
Tools for correlative cryo electron tomography
19. Grant Jensen (California Institute of Technology, USA)
How sample thickness and crowdedness affect interpretability in electron cryotomography
20. Radostin Danev (Okazaki Institute for Integrative Bioscience, Japan)
Zernike phase contrast for single particles and cryotomography
21. Gabriel Lander (The Scripps Research Institute, USA)
Appion: an integrated, database-driven pipeline for lucid EM image processing
22. Mark Ellisman (University of California San Diego, USA)
Multi-scale correlated light and electron microscopic imaging of the nervous system
23. Atsuo Miyazawa (RIKEN Harima Institute, Japan)
Development of a genetically encoded metalloprotein tag enabling protein detection by electron microscopy
24. Jiro Usukura (Nagoya University, Japan)
3D architecture of membrane cytoskeleton and spatial specificity of actin binding proteins revealed by immuno-freeze etching and cryo-microscopy
25. Winfried Denk (MPI-Heidelberg, Germany)
Reverse engineering the brain: tool to image activity and structure
26. Keiichi Namba (Osaka University, Japan)
Molecular mechanisms of self-assembly and protein export of the bacterial flagellum

WEDNESDAY, NOVEMBER 12
27. Kazuhiko Kinosita, Jr. (Waseda University, Japan)
Protein machines under an optical microscope
28. Robert Glaeser (University of California Berkeley, USA)
Towards nearly full-proteomic coverage in imaging of multiprotein complexes
29. Kuniaki Nagayama (Okazaki Institute for Integrative Bioscience/National Institute for Physiological Sciences, Japan)
A submicron design for an Aharonov-Bohm effect Hilbert differential phase plate
30. Michael Marko (Wadsworth Center, USA)
Technological improvements for biological cryo-TEM tomography
31. Rasmus Schröder (University of Heidelberg, Germany)
In-focus phase contrast by electrostatic phase plates in anamorphotic electron optics
32. Ueli Aebi (University of Basel, Switzerland)
The use of the atomic force microscope in the life sciences: opening new vistas for diagnosis, prevention and intervention
33. Jan Liphardt (University of California Berkeley, USA)
A superresolution view of the E. coli chemotaxis network
34. Takayuki Uchihashi (Kanazawa University, Japan)
High-speed AFM for visualizing biomolecular processes
35. Yuji Sasaki (Spring 8, Japan)
Dynamical single molecular observations on membrane proteins using X-rays and electrons
36. Hideo Higuchi (The University of Tokyo, Japan)
Imaging of stepwise motility of single motor molecules in living cells
37. Thomas Walz (Harvard Medical School, USA)
Electron microscopy of AQP0-mediated membrane junctions
38. Kaoru Mitsuoka (Japan Biological Information Research Center (AIST), Japan)
Structural analysis of membrane proteins and complexes by electron crystallography
39. Fred Sigworth (Yale University, USA)
Membrane proteins as single particles in cryo-EM
40. Chikara Sato (Neuroscience Research Institute (AIST), Japan)
Three-dimensional structures of ion channels, sensors and receptors revealed by single particle reconstruction
Closing address
(Kuniaki Nagayama, OIB/NIPS)


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