Monday, November 10th
9:30 - 9:40 Kuniaki Nagayama (National Institute for Physiological Science, Japan),
“Opening address”.
9:40 - 10:10 Tomomi Nemoto (National Institute for Physiological Science, Japan),
"Potential of two-photon microscopy for analysis of living organ”.
10:10 - 10:40 Kira Poskanzer (Columbia University, USA), “Development of two-photon
stimulation methods to map cortical circuits”.
10:40 - 11:10 Makio Tokunaga (National Institute of Genetics, Japan), "Highly inclined
thin illumination enables clear single-molecule imaging in linving cells".
11:10 - 11:40 Hiromi Okamoto (Institute for Molecular Science, Japan), "Potentiality
of Scanning Near-Field Optical Microscopy".
11:40 - 12:10 Susy Kohout (University of California, USA), "Probing Protein Motions
of Ci-VSP using Voltage Clamp Fluorometry".
12:10 - 13:00 Lunch
13:00 - 13:30 Wolfgang Baumeister (Max-Planck-Institute, Germany), "Cryoelectron
Tomography: Defining the Functional Modules of Cells".
13:30 - 14:00 Yasushi Hiraoka (Kobe Advanced ICT Research Center, Japan), “Correlative
light and electron microscopy for observing molecular dynamics in living cells”.
14:00 - 14:30 John Sedat (University of California, USA), “New Directions for Live
4-Dimensional Imaging Using OMX, a Novel Imaging Platform”.
14:30 - 15:00 Masataka Murakami (National Institute for Physiological Sciences, Japan),
"Salivary secretion: assessment of trans- and paracellular transport by physio-morphological
techniques".
15:00 - 15:30 Tomoko Nakanishi (Tokyo University, Japan), "Development of Radioisotope
Imaging Systems for Plants".
15:30 - 16:00 Haruo Sugi (Teikyo University, Japan), "Electron Microscopic Demonstration
of the Cross-bridge Recovery Stroke in Living Muscle Thick Filaments Using the Gas
Environment Chamber".
16:00 - 19:00 Poster session & buffet dinner
19:00 - 21:00 Selected poster presentations.
Tuesday, November 11th
9:30 - 10:00 Yoshiyuki Kubota (National Institute for Physiological Sciences, Japan),
"An excitatory and inhibitory synapse density on various GABAergic nonpyramidal cells
in the rat cerebral cortex".
10:00 - 10:30 Cedric Bouchet-Marquis (University of Colorado at Boulder, USA), "High
resolution imaging using CEMOVIS and cryo-ET".
10:30 - 11:00 Wah Chiu (Baylor College of Medicine, USA), "Backbone Tracing and
Model Building in Single Particle Cryo-EM".
11:00 - 11:30 Holger Stark (Max-Planck-Institute, Germany), "Studying 3D Dynamics
of Macromolecular Machines by Electron Cryomicroscopy".
11:30 - 12:00 Group photograph
12:00 - 13:00 Lunch
13:00 - 13:30 Takashi Ishikawa (ETH Zurich, Switzerland), "Molecular Arrangement
of Dynein in Flagella revealed by Cryo-Electron Tomography".
13:30 - 14:00 Abraham Koster (Leiden University Medical Center, The Netherlands),
"Tools for correlative cryo electron tomography".
14:00 - 14:30 Grant Jensen (California Institute of Technology, USA), "How sample
thickness and crowdedness affect interpretability in electron cryotomography".
14:30 - 15:00 Radostin Danev (Okazaki Institute for Integrative Bioscience, Japan),
"Zernike Phase Contrast for Single Particles and Cryotomography".
15:00 - 15:30 Gabriel Lander (The Scripps Research Institute, USA), "Appion: an
integrated, database-driven pipeline for lucid EM image processing".
15:30 - 16:00 Coffee break
16:00 - 16:30 Mark Ellisman (University of California San Diego, USA), "Multi-Scale
Correlated Light and Electron Microscopic Imaging of the Nervous System".
16:30 - 17:00 Atsuo Miyazawa (RIKEN Harima Institute, Japan), "Development of a
genetically encoded metalloprotein tag enabling protein detection by electron microscopy".
17:00 - 17:30 Jiro Usukura (Nagoya University, Japan), "3D Architecture of Membrane
Cytoskeleton and Spatial Specificity of Actin Binding Proteins Revealed by Immuno-freeze
Etching and Cryo-microscopy".
17:30 - 18:00 Winfried Denk (MPI-Heidelberg, Germany), "Reverse engineering the
brain: tool to image activity and structure".
18:00 - 18:30 Keiichi Namba (Osaka University, Japan), "Molecular Mechanisms of
Self-Assembly and Protein Export of the Bacterial Flagellum".
18:45 - 21:00 Banquet
Wednesday, November 12th
9:30 - 10:00 Kazuhiko Kinosita, Jr. (Waseda University, Japan), "Protein Machines
under an Optical Microscope".
10:00 - 10:30 Robert Glaeser (University of California Berkeley, USA), "Towards
Nearly Full-Proteomic Coverage in Imaging of Multiprotein Complexes".
10:30 - 11:00 Kuniaki Nagayama (Okazaki Institute for Integrative Bioscience, Japan),
"A Submicron Design for an Aharonov-Bohm Effect Hilbert Differential Phase Plate".
11:00 - 11:30 Michael Marko (Wadsworth Center, USA), "Technological Improvements
for Biological Cryo-TEM tomography".
11:30 - 12:00 Rasmus Schroeder (University of Heidelberg, Germany), "In-focus phase
contrast by electrostatic phase plates in anamorphotic electron optics".
12:00 - 13:00 Lunch
13:00 - 13:30 Ueli Aebi (University of Basel, Switzerland), "The use of the atomic
force microscope in the life sciences: opening new vistas for diagnosis, prevention
and intervention".
13:30 - 14:00 Jan Liphardt (University of California Berkeley, USA), "A Superresolution
View of the E. coli Chemotaxis Network".
14:00 - 14:30 Takayuki Uchihashi (Kanazawa University, Japan), "High-Speed AFM for
Visualizing Biomolecular Processes".
14:30 - 15:00 Yuji Sasaki (Spring 8, Japan), "Dynamical Single Molecular Observations
on Membrane Proteins Using X-Rays and Electrons".
15:00 - 15:30 Hideo Higuchi (Tokyo University, Japan), "Imaging of stepwise motility
of single motor molecules in living cells".
15:30 - 16:00 Coffee break
16:00 - 16:30 Thomas Walz (Harvard Medical School, USA), "Electron Microscopy of
AQP0-mediated Membrane Junctions".
16:30 - 17:00 Kaoru Mitsuoka (Japan Biological Information Research Center (AIST), Japan),
"Structural analysis of membrane proteins and complexes by electron crystallography".
17:00 - 17:30 Fred Sigworth (Yale University, USA), "Membrane proteins as single
particles in cryo-EM".
17:30 - 18:00 Chikara Sato (Neuroscience Research Institute (AIST), Japan), "Three-Dimensional
Structures of Ion Channels, Sensors and Receptors Revealed by Single Particle Reconstruction".
18:00 - 18:10 Kuniaki Nagayama (National Institute for Physiological Science, Japan),
“Closing address”.
