ES細胞を用いたキメラマウス作製法 [D-5] - 生理学実験技術データベース

D-5 Last update: 2010-03-31

ES細胞を用いたキメラマウス作製法

The method of producing the chimeric mouse with embryonic stem cells

Summary	The mouse that modifies the mouse chromosome freely according to the purpose can be made as a method of producing the mouse that modifies the gene in the gene targeting mouse producing system that use embryonic stem cells (ES cells) though there are a method of injecting DNA directly into the fertilized egg and using the virus. This method was established to the technology that made the chimeric mouse by using ES cells that had been established in the 1970's by combining with the technology that did the homologous recombination to ES cells. ES cells cultured with the early embryo differentiates under normal embryo generation, and forms the chimeric mouse composed of the host embryo cell. Because ES cells differentiates into chimeric mouse's germ-line cells, the gene mutation of ES cells is inherited to the descendant mouse since the next generation by mating of the chimeric mouse and the wild-type mouse, and a new strain of mouse is created.
内容	基本的なマウス胚操作のための技術を各項目ごとに解説する。それらの技術を用いたES細胞導入によるキメラマウス作製について説明する。・序文・図１：ジーンターゲティングマウス作製の原理・図２：マイクロインジェクション法による8細胞期胚へのES細胞の導入（左上）8細胞期胚、（右上）ES細胞を吸っているところ、（左下）ES細胞を導入しているところ、（右下）胚盤胞・図３： ES細胞を初期胚に導入して作製したキメラマウス・図４：作成手順
特徴	ES細胞のマウス8細胞期胚およびマウス胚盤胞へのマイクロインジェクション法によるキメラマウスの作製
主な試薬・機器	各項目ごとに記述予定