• 根本研究室

2022

原著論文 Paper

Ryu Hashimoto, Masafumi Minoshima, Souhei Sakata, Fumihito Ono, Hirokazu Ishii, Yuki Watakabe, Tomomi Nemoto, Saeko Yanaka, Koichi Kato and Kazuya Kikuchi
"Efficient visible/NIR light-driven uncaging of hydroxylated thiazole orange-based caged compounds in aqueous media"
Chemical Science (2022) 13:7462-7467, Published: 02 Jun 2022

Ching-Pu Chang, Kohei Otomo, Yuichi Kozawa, Hirokazu Ishii, Miwako Yamasaki, Masahiko Watanabe, Shunichi Sato, Ryosuke Enoki, Tomomi Nemoto
"Single-scan volumetric imaging throughout thick tissue specimens by one-touch installable light-needle creating device"
Scientific Reports (2022) 12:10468 , Published: 21 June 2022

Takafumi Kamada, Kohei Otomo, Takashi Murata, Kaito Nakata, Shota Hiruma, Ryota Uehara, Mitsuyasu Hasebe, Tomomi Nemoto
"Low-invasive 5D visualization of mitotic progression by two-photon excitation spinning-disk confocal microscopy"
Scientific Reports (2022) 12:809 , Published: 17 January 2022

総説 review

概日時計中枢におけるカルシウムリズム
榎木亮介, 生物物理 62(4) 228-231, 2022年8月

二光子励起顕微鏡法によるプレシナプス構成因子の可視化
石井宏和, 根本知己, Clinical Neuroscience 40(6):731-735, 2022年6月

二光子顕微鏡法の高解像化・高速化への展開
大友康平, 山口和志, 石井宏和, 根本知己, 生物物理 62(2) 131-133, 2022年3月

新規的な技術を用いた二光子顕微鏡による生体イメージングの展開
根本知己, 大友康平, 山口和志, 高橋泰伽, レーザー研究 50(27) 68-71, 2022年2月

何ができる?どこで使える?先端蛍光顕微鏡使い分けガイド
大友康平, 野中茂樹, 亀井保博, 実験医学 40(1) 67-76, 2022年1月

2021

原著論文 Paper

Hirokazu Ishii, Kohei Otomo, Taiga Takahashi, Kazushi Yamaguchi, Tomomi Nemoto,
"Focusing new light on brain functions: multiphoton microscopy for deep and super-resolution imaging" Neuroscience Research, Accepted:30 November 2021

Tomoko Kamasaki, Yumi Miyazaki, Susumu Ishikawa, Kazuya Hoshiba, Keisuke Kuromiya, Nobuyuki Tanimura Yusuke Mori, Motosuke Tsutsumi, Tomomi Nemoto, Ryota Uehara, Shiro Suetsugu, Toshiki Itoh, Yasuyuki Fujita
"FBP17-mediated finger-like membrane protrusions in cell competition between normal and RasV12-transformed cells" iScience 24(9), 102994, DOI:10.1016/j.isci.2021.102994, Published:24 September 2021 (Available online 18 August 2021)

Taiga Takahashi, Hong Zhang, Kohei Otomo, Yosuke Okamura, Tomomi Nemoto,
"Protocol for constructing an extensive cranial window utilizing a PEO-CYTOP nanosheet for in vivo wide-field imaging of the mouse brain" STAR Protocols 2(2), DOI:10.1016/j.xpro.2021.100542, Published:18 June 2021

N.Kon, H.Wang, Y.Kato, K.Uemoto, N.Kawamoto, K.Kawasaki, R.Enoki, G.Kurosawa, T.Nakane, Y.Sugiyama, H.Tagashira, M.Endo, H.Iwasaki, T.Iwamoto, K.Kume, Y.Fukada.
"Na+/Ca2+ exchanger mediates cold Ca2+ signaling conserved for temperature-compensated circadian rhythms" Science Advances 7, DOI: 10.1126/sciadv.abe8132, Published:30 April 2021

Titinunt Kitrungrotsakul, Xian-Hua Han, Yutaro Iwamoto, Satoko Takemoto, Hideo Yokota, Sari Ipponjima, Tomomi Nemoto, Wei Xiong, Yen-Wei Chen
"A Cascade of 2.5D CNN and Bidirectional CLSTM Network for Mitotic Cell Detection in 4D Microscopy Image" IEEE ACM Trans. Comput. Biol. Bioinform. 18(2), DOI:10.1109/TCBB.2019.2919015, Published:1 April 2021

Ming-Liang Lee, Hirokazu Matsunaga, Yuki Sugiura, Takahiro Hayasaka, Izumi Yamamoto, Taiga Ishimoto, Daigo Imoto, Makoto Suematsu, Norifumi Iijima, Kazuhiro Kimura, Sabrina Diano, Chitoku Toda
"Prostaglandin in the ventromedial hypothalamus regulates peripheral glucose metabolism", Nature Communications 12 (2021) DOI: 10.1038/s41467-021-22431-6, Published:20 April 2021

Titinunt Kitrungrotsakul, Yutaro Iwamoto, Satoko Takemoto, Hideo Yokota, Sari Ipponjima, Tomomi Nemoto, Lanfen Lin, Ruofeng Tong, Jingsong Li, Yen-Wei Chen
"Accurate and fast mitotic detection using an anchor-free method based on full-scale connection with recurrent deep layer aggregation in 4D microscopy images" BMC bioinformatics 22(1), doi:10.1186/s12859-021-04014-w, Published: 26 February 2021

Chentao Wen, Takuya Miura, Venkatakaushik Voleti, Kazushi Yamaguchi,Motosuke Tsutsumi, Kei Yamamoto, Kohei Otomo, Yukako Fujie, TakayukiTeramoto, Takeshi Ishihara, Kazuhiro Aoki, Tomomi Nemoto, ElizabethM.C. Hillman, Koutarou D. Kimura
3DeeCellTracker, a deep learning-based pipeline for segmenting and tracking cells in 3D time lapse images eLife vol.10  pp:59187-1 - 59187-36 (2021) DOI: 10.7554/eLife.59187

GABA from vasopressin neurons regulates the time at which suprachiasmatic nucleus molecular clocks enable circadian behavior. T. Maejima, Y.Tsuno, S.Miyazaki, Y.Tsuneoka, E.Hasegawa, M.Islam, R.Enoki, T.Nakamura, M.Mieda, PNAS 118 (6) e2010168118, February 9, 2021

Dynamic organization of cortical actin filaments during the ooplasmic segregation of ascidian Ciona eggs , Hirokazu Ishii and Tomomi Tani, Molecular Biology of the Cell,Vol. 32, No. 3,(Published Online:28 Jan 2021)

Optical clearing of living brains with MAGICAL to extend in vivo imaging , Kouichirou Iijima,Takuto Oshima,Ryosuke Kawakami,Tomomi Nemoto, iScience,Volume 24, Issue 1, January 22 2021 (Available online 3 December 2020)

総説 review

二光子顕微鏡法の技術展開と生体イメージング応用
大友康平, 根本知己, OPTRONICS 40(12):99-103. 2021年12月

二光子顕微鏡法の機能向上と生理学応用
大友康平, 根本知己, レーザー学会第557回研究報告 RTM-21(27):11-16, 2021年10月

生命多光子顕微鏡による深部高解像・超解像イメージング
大友康平, 髙橋泰伽, 山口和志, 石井宏和, 根本知己, 光学 50(4):141-146, 2021年4月

2020

[Articles]

Autophagy contributes to quality control of leaf mitochondria, Sakuya Nakamura, Shinya Hagihara, Kohei Otomo, Hiroyuki Ishida, Jun Hidema, Tomomi Nemoto, Masanori Izumi, Plant and Cell Physiology, 1 February 2021 (online 23 December 2020)

Adaptive Optical Two Photon Microscopy for Surface Profiled Living Biological Specimens, Kazushi Yamaguchi, Kohei Otomo, Yuichi Kozawa, Motosuke Tsutsumi, Tomoko Inose, Kenji Hirai, Shunichi Sato, Tomomi Nemoto*, and Hiroshi Uji-i*, ACS Omega, 6(1) 438-447, 12 January (2021),(online 30 November 2020)

PEO-CYTOP Fluoropolymer Nanosheets as a Novel Open-Skull Window for Imaging of the Living Mouse Brain, Taiga Takahashi, Hong Zhang, Ryosuke Kawakami, Kenji Yarinome, Masakazu Agetsuma, Junichi Nabekura, Kohei Otomo, Yosuke Okamura, Tomomi Nemoto, iScience, VOLUME 23, ISSUE 10, 101579, OCTOBER 23, 2020 (Open Access Published:September 21, 2020)

In vivo two-photon microscopic observation and ablation in deeper brain regions realized by modifications of excitation beam diameter and immersion liquid, Kazushi Yamaguchi, Ryoji Kitamura, Ryosuke Kawakami, Kohei Otomo, Tomomi Nemoto, PLOS ONE, 15(8): e0237230, August 7, 2020

High-peak-power 918-nm laser light source based two-photon spinning-disk microscopy for green fluorophores, Kohei Otomo, Ai Goto, Yumi Yamanaka, Takashi Hori, Hiroshi Nakayama, Tomomi Nemoto, Biochemical and Biophysical Research Communications, 529(2) 238-242, 2020

Absorption, Fluorescence, and Two-Photon Excitation Ability of 5-Phenylisolidolo[2,1-a]quinolines Ramon Francisco Avena, Lin Qiao, Yuki Fujii, Kohei Otomo, Hirokazu Ishii, Takeyuki Suzuki, Hirofumi Tsujino, Tadayuki Uno, Yasuo Tsutsumi, Yusuke Kawashima, Tatsuya Takagi, Kenichi Murai, Tomomi Nemoto, Mitsuhiro Arisawa ACS Omega 5(5) 2473 - 2479

Nanosheet wrapping-assisted coverslip-free imaging for looking deeper into a tissue at high resolution , Hong Zhang, Kenji Yarinome, Ryosuke Kawakami, Kohei Otomo, Tomomi Nemoto, Yosuke Okamura, PLOS ONE 15(1): e0227650-1- e0227650-16. doi:10.1371/journal.pone.0227650, January 10, 2020

2019

[Articles]

A Novel Katanin-Tethering Machinery Accelerates Cytokinesis, Takema Sasaki, Motosuke Tsutsumi, Kohei Otomo, Takashi Murata, Noriyoshi Yagi, Masayoshi Nakamura, Tomomi Nemoto, Mitsuyasu Hasebe, Yoshihisa Oda, Current Biology 29(23) 4060 - 4070

Nonmuscle myosin IIA and IIB differently suppress microtubule growth to stabilize cell morphology, 4. Sato Y, Kamijo K, Tsutsumi M, Murakami Y, Takahashi M, Journal of Biochemistry, Volume 167, Issue 1, January 2020, Pages 25–39 Published: 10 October 2019

An end-to-end CNN and LSTM network with 3D anchors for mitotic cell detection in 4D microscopic images and its parallel implementation on multiple GPUs , Titinunt Kitrungrotsakul, Xian-Hua Han, Yutaro Iwamoto, Satoko Takemoto, Hideo Yokota, Sari Ipponjima, Tomomi Nemoto, Wei Xiong & Yen-Wei Chen, Neural Computing and Applications, 32:5669–5679, Published: 02 August 2019

587 nm nanosecond optical pulse generation by synchronously-driven gain-switched laser diodes with optical injection locking , Jui-Hung Hung, Yuan Gao, Hejie Yan, Kazuo Sato, Hirohito Yamada, Lung-Han Peng, Tomomi Nemoto, Hiroyuki Yokoyama, App. Phys. Express(2019), vol. 12, No. 8, pp: 082002-1 - 082002-5, Published 9 July 2019

Two-photon STED nanoscopy realizing 100-nm spatial resolution utilizing high-peak-power sub-nanosecond 655-nm pulses, Hirokazu Ishii, Kohei Otomo, Jui-Hung Hung, Motosuke Tsutsumi, Hiroyuki Yokoyama, and Tomomi Nemoto, Biomedical Optics Express, 10, 3104-3113 (2019)

Rational engineering of XCaMPs, a multicolor GECI suite for in vivo imaging of complex brain circuit dynamics , M. Inoue, A. Takeuchi, S. Manita., S. Horigane., M. Sakamoto, R. Kawakami, K.Yamaguchi, K. Otomo, H. Yokoyama, R. Kim, T. Yokoyama, S. Takemoto-Kimura, M. Abe, M. Okamura, Y. Kondo, S. Quirin, C. Ramakrishnan, T. Imamura, K. Sakimura, T. Nemoto, M. Kano, H. Fujii, K. Deisseroth, K. Kitamura, H. Bito, Cell, 2019

"A Cascade of 2.5D CNN and Bidirectional CLSTM Network for Mitotic Cell Detection in 4D Microscopy Image.", Kitrungrotsakul T, Han XH, Iwamoto Y, Takemoto S, Yokota H, Ipponjima S, Nemoto T, Wei X, Chen YW, IEEE/ACM transactions on computational biology and bioinformatics

"Real-Time Polarization-Resolved Imaging of Living Tissues Based on Two-Photon Excitation Spinning Disk Confocal Microscopy", Ai Goto, Kohei Otomo, Tomomi Nemoto, Frontiers in Physics 7 56-1 - 56-9

"Differential contributions of nonmuscle myosin IIA and IIB to cytokinesis in human immortalized fibroblasts.", Yamamoto K, Otomo K, Nemoto T, Ishihara S, Haga H, Nagasaki A, Murakami Y, Takahashi M, Experimental cell research 376(1) 67 - 76

Optical clearing of living brains with MAGICAL to extend in vivo imaging, Kouichirou Iijima, Takuto Oshima, Ryosuke Kawakami, Tomomi Nemoto, BioXriv, 507426 (2019) doi: 10.1101/507426

2018

Heterogeneous distribution of doublecortin-expressing cells surrounding the rostral migratory stream in the juvenile mouse , Y. Aoyagi, T. Hibi, Y. Kimori, M. Sawada, R.Kawakami, K. Sawamoto, T.Nemoto, J. Comparative Neurol. , 2018, 526, 2631-2646

A 2.5D Cascaded Convolutional Neural Network with Temporal Information for Automatic Mitotic Cell Detection in 4D Microscopic Images, Titinunt Kitrungrotsakul, Xian-Hau Han, Yutaro Iwamoto, Satoko Takemoto, Hideo Yokota, Sari Ipponjima, Tomomi Nemoto, Xiong Wei and Yen-Wei Chen the 2018 14th International Conference on Natural Computation, Fuzzy Systems and Knowledge Discovery (ICNC-FSKD 2018) doi:10.1109/FSKD.2018.8687125

Development of 3D imaging technique of reconstructed human epidermis with immortalized human epidermal cell line, Inoue, Yu; Hasegawa, Seiji; Miyachi, Katsuma; Yamada, Takaaki; Nakata, Satoru; Ipponjima, Sari; Hibi, Terumasa; Nemoto, Tomomi ; Tanaka, Masahiko; Suzuki, Ryo; Hirashima, Naohide, Experimental Dermatology (2018)

Advanced easySTED microscopy based on two-photon excitation by electrical modulations of light pulse wavefronts., K. Otomo, T. Hibi, Y.-C. Fang, J.-H. Hung, M, Tsutsumi,, R. Kawakami,, H. Yokoyama, T. Nemoto. Biomed. Opt. Express, (2018) Vol. 9, Iss. 6, pp: 2476-2680

Super-resolution structural analysis of dendritic spines using three-dimensional structured illumination microscopy in cleared mouse brain slices, Kazuaki Sawada, Ryosuke Kawakami, Ryuichi Shigemoto, Tomomi Nemoto, Eur. J. Neurosci. (2018) 47(9):1033-1042

Improvement of two-photon microscopic imaging in deep regions of living mouse brains by utilizing a light source based on an electrically controllable gain-switched laser diode, Kazuaki Sawada, Ryosuke Kawakami, Yi-Cheng Fang, Jui-Hung Hung, Yuichi Kozawa, Kohei Otomo, Shunichi Sato, Hiroyuki Yokoyama, Tomomi Nemoto, Proc. SPIE, p. 104982L-1 - 104982L-10 (2018) doi:10.1117/12.2288664

2017

Jui-Hung Hung, Kazuo Sato, Yi-Cheng Fang, Lung-Han Peng, Tomomi Nemoto,Hiroyuki Yokoyama
"Generation of high-peak-power sub-nanosecond 650-nm band optical pulses based on semiconductor laser controlling technologies"
Appl. Phys. Express vol. 10, No. 10, pp. 102701-1 - 102701-4 (2017) doi:10.7567/APEX.10.102701

Shota Hiruma, Tomoko Kamasaki, Kohei Otomo, Tomomi Nemoto, and Ryota Uehara
"Dynamics and function of ERM proteins during cytokinesis in human cells"
FEBS Lett., vol. 591, No. 20, pp. 3296-3309, doi: 10.1002/1873-3468.12844, 20 September 2017

Hong Zhang, Ami Masuda, Ryosuke Kawakami, Kenji Yarinome, Riku Saito, Yu Nagase, Tomomi Nemoto,, Yosuke Okamura
“Fluoropolymer nanosheet as a wrapping mount for high quality tissue imaging”
Advanced Materials vol. 29, pp. 1703139-1-1703139-6 (2017) DOI: 10.1002/adma.201703139, Published: Aug. 11

2016

Masataka Kunii, Mica Ohara-Imaizumi, Noriko Takahashi, Masaki Kobayashi, Ryosuke Kawakami, Yasumitsu Kondoh, Takeshi Shimizu, Siro Simizu, Bangzhong Lin, Kazuto Nunomura, Kyota Aoyagi, Mitsuyo Ohno, Masaki Ohmuraya, Takashi Sato, Shin-ichiro Yoshimura, Ken Sato, Reiko Harada, Yoon-Jeong Kim, Hiroyuki Osada, Tomomi Nemoto, Haruo Kasai, Tadahiro Kitamura, Shinya Nagamatsu, and Akihiro Harada
“The opposite roles of SNAP23 in the exocrine and endocrine pancreas”
J. Cell Biol. vol. 215(1), pp. 121- 138,(2016) DOI: 10.1083/jcb.201604030

Ayano Tanabe, Terumasa Hibi, Sari Ipponjima, Kenji Matsumoto, Masafumi Yokoyama, Makoto Kurihara, Nobuyuki Hashimoto, Tomomi Nemoto
“Transmissive liquid crystal device for correcting primary coma aberration and astigmatism in biospecimen in two-photon excitation laser scanning microscopy”,
J. Biomed. Opt. vol. 21, No. 12, 121503-1 - 121503-10, (2016) doi: 10.1117/1.JBO.21.12.121503

Ayano Tanabe, Terumasa Hibi, Sari Ipponjima, Kenji Matsumoto, Masafumi Yokoyama, Makoto Kurihara, Nobuyuki Hashimoto, Tomomi Nemoto
“Transmissive liquid-crystal device correcting primary coma aberration and astigmatism in laser scanning microscopy”,
Proc. SPIE, p . 9717-1 (2016)  doi:10.1117/12.2209459

2015

Ayano Tanabe, Terumasa Hibi, Sari Ipponjima, Kenji Matsumoto, Masafumi Yokoyama, Makoto Kurihara, Nobuyuki Hashimoto, Tomomi Nemoto
”Correcting spherical aberrations in biospecimen using transmissive liquid crystal device in two-photon excitation laser scanning microscopy”
J. Biomed. Opt. 20(10), pp: 101204-1 – 101204-11 (2015)

Kohei Otomo, Terumasa Hibi, Takashi Murata, Hirotaka Watanabe, Ryosuke Kawakami, Hiroshi Nakayama, Mitsuyasu Hasebe, Tomomi Nemoto
“Multi-point scanning two-photon excitation microscopy utilising a high-peak-power 1042-nm laser”
Anal. Sci. Vol. 31, No. 4, pp. 307-313. (2015) 表紙に採用, Hot Article Award受賞

Ryosuke Kawakami, Kazuaki Sawada, Yuta Kusama, Yi-Cheng Fang, Shinya Kanazawa, Yuichi Kozawa, Shunichi Sato, Hiroyuki Yokoyama and Tomomi Nemoto
“In vivo two-photon imaging of mouse hippocampal neurons in dentate gyrus using a light source based on a high-peak power gain-switched laser diode”
Biomed. Opt. Express Vol. 6, Issue 3, pp. 891-901 (2015)

Tanabe A, Hibi T, Matsumoto K, Yokoyama M, Kurihara M, Ipponjima S, Hashimoto N, Nemoto T.
Transmissive liquid crystal devices correcting the spherical aberrations in laser scanning microscopy
Proc. SPIE Bios. p. 9335-1,(2015) doi:10.1117/12.2076342

Yuka Aoyagi, Ryosuke Kawakami, Hisayuki Osanai, Terumasa Hibi, Tomomi Nemoto
A Rapid Optical Clearing Protocol using 2,2-Thiodiethanol for Microscopic Observation of Fixed Mouse Brain”,
PLoS ONE 10(1): e0116280. doi:10.1371/journal.pone.0116280 被引用回数トップ25% (207.7.4)

M. Nakao, S. Takemoto, T. Sugiura, K. Sawada, R. Kawakami, T. Nemoto and T. Matsuda
"Interactive Visual Exploration of Overlapping Similar Structures for Three-dimensional Microscope Images",
BMC Bioinformatics 15(1):415. (2015) doi: 10.1186/s12859-014-0415-x

2014

Megumi Nakao*†, Shintaro Takemoto†, Tadao Sugiura†, Kazuaki Sawada†, Ryosuke Kawakami†, Tomomi Nemoto† and Tetsuya Matsuda
"Interactive Visual Exploration of Overlapping Similar Structures for Three-dimensional Microscope Images" BMC Bioinformatics vol. 15, article# 415 (2014) doi:10.1186/s12859-014-0415-x

Yuka Aoyagi, Ryosuke Kawakami, Hisayuki Osanai, Terumasa Hibi, Tomomi Nemoto
“A Rapid Optical Clearing Protocol using 2,2’-Thiodiethanol for Microscopic Observation of Fixed Mouse Brain” PLoS ONE vol. 10, No. 1, Article#: e0116280. e0116280-1 - e0116280-13 doi:10.1371/journal.pone.0116280

Kohei Otomo†, Terumasa Hibi†, Yuichi Kozawa,Makoto Kurihara,Nobuyuki Hashimoto, Hiroyuki Yokoyama,Shunichi Sato, Tomomi Nemoto*
“Two-Photon Excitation STED Microscopy by Utilizing Transmissive Liquid Crystal Devices” Optics Expressvol. 22, No, 23, pp. 28215-28221 (2014) †equally contributed

Tomomi Nemoto
“Development of novel two-photon microscopy for living brain and neuron” Microscopy vol. 63 (suppl 1), pp. i7-i8 (2014) doi: 10.1093/jmicro/dfu087

Yusuke Oshima, Takaeshi Imamura, Atsuko Shintani, Hiroko Kajiura-Kobayashi, Terumasa Hibi, Takeharu Nagai, shigenori Nonaka, Tomomi Nemoto
“Ultrasensitive imaging of Ca2+ dynamics in pancreatic acinar cells of Yellow Cameleon-Nano transgenic mice” Int. J. Mol. Sci. vol. 15, No. 11, pp: 19971-86.

Megumi Nakao, Kosuke Kurebayashi, Tadao Sugiura, Tetsuo Sato, Kazuaki Sawada, Ryosuke Kawakami, Tomomi Nemoto, Kotaro Minato, Tetsuya Matsuda
“Visualizing in vivo Brain Neural Structures Using Volume Rendered Feature Spaces” Computers in Biology and Medicine vol. 53, pp. 85-93. (2014) doi: 10.1016/j.compbiomed.2014.07.007

Satoshi Asano, Tomomi Nemoto, Tomoya Kitayama, Kae Harada, Jun Zhang, Kana Harada, Isei Tanida, Masato Hirata, and Takashi, Kanematsu
“Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion” Biology Open vol. 3, No. 6, pp. 463-474. (2014) doi: 10.1242/bio.20147591

Yuta Kusama,Yuichiro Tanushi,Masami Yokoyama,Ryosuke Kawakami,Terumasa Hibi,Yuichi Kozawa,Tomomi Nemoto,Shunichi Sato,and Hiroyuki Yokoyama*
“7-ps optical pulse generation from a 1064-nm gain-switched laser diode and its application for two-photon microscopy”
Optics Express, vol. 22, No. 5, pp. 5746-5753 (2014) doi: 10.1364/OE.22.005746

Sari Ipponjima, Terumasa Hibi, Yuichi Kozawa, Hibiki Horanai, Hiroyuki Yokoyama, Shunichi Sato, Tomomi Nemoto
"Improvement of lateral resolution and extension of depth of field in two-photon microscopy by a higher-order radially polarized beam"
Microscopy, vol. 63, No. 1, pp. 23-32. (2014) doi: 10.1093/jmicro/dft041

2013

Daisuke Takao, Tomomi Nemoto, Takaya Abe, Hiroshi Kiyonari, Hiroko Kajiura-Kobayashi, Hidetaka Shiratori, Shigenori Nonaka
”Asymmetric distribution of dynamic calcium signals in the node of mouse embryo during left–right axis formation”
Developmental Biol, vol. 376, No.1, pp. 23-30. (2013) doi: 10.1016/j.ydbio.2013.01.018

Ryosuke Kawakami, Kazuaki Sawada, Aya Sato, Terumasa Hibi, Yuichi Kozawa, Shunichi Sato, Hiroyuki Yokoyama, and Tomomi Nemoto
"Visualizing hippocampal neurons with in vivo two-photon microscopy using a 1030 nm picosecond pulse laser"
Scientific Rep., vol. 3, Article #1014. pp: 1014-1 – 1014-7(2013) doi:10.1038/srep01014

2012

Sho Ogata, Takashi Miki, Susumu Seino, Seiichi Tamai, Haruo Kasai, Tomomi Nemoto
”A Novel Function of Noc2 in Agonist-Induced Intracellular Ca2+ Increase during Zymogen-Granule Exocytosis in Pancreatic Acinar Cells”
PLoS ONE, 7(5): e37048. doi:10.1371/journal.pone.0037048

Ayano Tanabe, Yuka Saito, Makoto Kurihara, Nobuyuki Hashimoto,Yuichi Kozawa, Shunichi Sato, Terumasa Hibi, Tomomi Nemoto
“Observation of PDLCs by SHG laser scanning microscopy using a liquid crystal vector beam generator”
Proc SPIE, p. 8279-20 (2012) doi:10.1117/12.907076

2011

Hiroyuki Inada, Miho Watanabe, Taku Uchida, Hitoshi Ishibashi, Hiroaki Wake, Tomomi Nemoto, Yuchio Yanagawa, Atsuo Fukuda, Junichi Nabekura
“GABA regulates the multidirectional tangential migration of GABAergic interneurons in living neonatal mice”
PLoS ONE, Vol. 6, No. 12, e27048 (epub)(2011) doi:10.1371/journal.pone.0027048

Yuichi Kozawa†, Terumasa Hibi†, Aya Sato, Hibiki Horanai, Makoto Kurihara, Nobuyuki Hashimoto, Hiroyuki Yokoyama, Tomomi Nemoto, and Shunichi Sato (†: equally contributed)
"Lateral resolution enhancement of laser scanning microscopy by higher-order radially polarized mode beam"
Optics Express, Vol. 19, Issue 17, pp. 15947-15954 (2011) doi: 10.1364/OE.19.015947

Masato Sawada, Naoko Kaneko, Hiroyuki Inada, Hiroaki Wake, Yasuko Kato, Yuchio Yanagawa, Kazuto Kobayashi, Tomomi Nemoto, Junichi Nabekura, and Kazunobu Sawamoto
"Sensory input regulates spatial and subtype-specific patterns of neuronal turnover in the adult olfactory bulb"
J. Neurosci., vol.31, pp. 11587-11596 (2011) doi: 10.1523/JNEUROSCI.0614-11.2011

2010

Yukihiko Ebisuno, Koko Katagiri, Tomoya Katakai, Yoshihiro Ueda, Tomomi Nemoto, Hiroyuki Inada, Junichi Nabekura, Takaharu Okada, Reiji Kannagi, Toshiyuki Tanaka, Masayuki Miyasaka, Nancy Hogg,* Tatsuo Kinashi
“Rap1 controls lymphocyte adhesion cascade and interstitial migration within lymph nodes in a RAPL-dependent and -independent manners”
Blood, 115(4):804-14 (2010) doi:10.1182/blood-2009-03-211979

Tomomi Nemoto
"Potential of “in vivo” Two-photon microscopy in visualization of 3D structure and functional information in living body”
J. Pharmacolog. Sci., vol.112, p. 45P (2010)

Tomomi Nemoto
“Two-photon microscopic analysis of sequential compound exocytosis”
J. Phyisiol. Sci., vol. 60, p. S45-S45 (2010)

2009

Naoto Matsuda, Hui Lu, Yuko Fukata, Jun Noritake, Hongfeng Gao, Sujay Mukherjee, Tomomi Nemoto, Masaki Fukata, Mu-ming Poo
“Differential Activity-Dependent Secretion of Brain-Derived Neurotrophic Factor from Axon and Dendrite”
J. Neurosci., 29(45), 14185-98 (2009)

Eriko Ohta, Tomohiro Itoh, Tomomi Nemoto, Jiro Kumagai, Shigeru B.H. Ko, Kenichi Ishibashi, Mayuko Ohno, Keiko Uchida, Akihito Ohta, Eisei Sohara, Shinichi Uchida, Sei Sasaki, *Tatemitsu Rai
“Pancreas-specific aquaporin 12 null mice showed increased susceptibility to caerulein-induced acute pancreatitis”
Am. J. Physiol. Cell Physiol., 297(6):C1368-78 (2009) doi: 10.1152/ajpcell.00117.2009

Yusuke Takatsuru, Dai Fukumoto, Miki Yoshitomo, Tomomi Nemoto, Hideo Tsukada, *Junichi Nabekura
“Neuronal circuit remodeling in the contralateral cortical hemisphere during functional recovery from cerebral infarction”
J. Neurosci., vol.29, pp.10081-6 (2009) doi:10.1523/JNEUROSCI.1638-09.2009

Wataru Tomosugi, Tomoki Matsuda, Tomomi Tani, Ippei Kotera, Kenta Saito, Kazuki Horikagwa, Tomomi Nemoto, and *Takeharu Nagai
“An ultramarine fluorescent protein with high photostability and pH insensitivity”
Nature Methods, vol.6, pp.351-353 (2009) doi: 10.1038/NMETH.1317

Takashi Kanematsu, Tomomi Nemoto, Masato Hirata
“Deficiency of PRIP gene in mice exhibits a phenotype of hyperinsulinemia”
J. Pharmacolog. Sci., vol.111, p. 45P, (2009)

Masato Sawada, Naoko Kaneko, Hiroaki Wake, Hiroyuki Inada, Yasuko Kato, Yuchio Yanagawa, Kazuto Kobayashi, Tomomi Nemoto, Junichi Nabekura, and Kazunobu Sawamoto
“In vivo imaging of sensory input-dependent neurogenesis in the adult olfactory bulb”
Neurosci. Res., vol. 65, p. S54-S54 (2009) doi: 10.1016/j.neures.2009.09.130

Takatsuru Y, Fukumoto D, Yoshitomo M, Nemoto T, Tsukada H, Nabekura J.
“The functional remodeling of contralateral hemisphere during the recovery from the focal infarction in somatosensory cortex”
J. Phyisiol. Sci., vol. 59, p. 372-372 (2009)

Eriko Ohta, Tomohiro Itoh, Tomomi Nemoto, Jiro Kumagai, Shigeru B.H. Ko, Kenichi Ishibashi, Mayuko Ohno, Keiko Uchida, Akihito Ohta, Eisei Sohara, Shinichi Uchida, Sei Sasaki, Tatemitsu Rai
“Pancreas-specific aquaporin12 null mice showed increased susceptibility to caerulein-induced acute pancreatitis”
J. Phyisiol. Sci., vol. 59, p. 399-399 (2009)

Takashi Kanematsu, Tomomi Nemoto, Masato Hirata Masato
“PRIP-KO mice exhibit a phenotype of hyperinsulinemia”
J. Pharmocolg. Sci., vol. 109 p. 92P-92P (2009)

2008

Tomomi Nemoto
“Principle and potential of two-photon microscopy”
Neurosci. Res.vol. 61, p. S27-S27 (2008)

2007

*Chiming Wei, Takeharu Nagai, Wenchi Wei, Tomomi Nemoto, Muhammad Awais, Osamu Niwa, Ryoji Kurita, Yashinobu Baba
“New advances in Nanomedicine: Diagnosis and Preventive Medicine”
Med. Clin. N. Am., vol.91, pp.871-879 (2007) doi: 10.1016/j.mcna.2007.05.002

Hiroaki Wake, Tomomi Nemoto, and Junichi Nabekura,
“Long term observation of fine structural plasticity of neurons and glias in damaged cerebral cortex of living mice”
Neurosci. Res., vol. 58, p. S12-S12 (2007) doi: 10.1016/j.neures.2007.06.070

2006

*Haruo Kasai, Takuya Kishimoto , Tomomi Nemoto , Hiroyasu Hatakeyama , Ting-Ting Liu , Noriko Takahashi
“Two-photon excitation imaging of exocytosis and endocytosis and their spatial organizations”
Adv. Drug Deliv. Rev., vol.58, pp.850-77 (2006) doi: 10.1016/j.addr.2006.07.008

Takuya Kishimoto, Ryoichi Kimura, Ting-Ting Liu, Tomomi Nemoto, Noriko Takahashi and *Haruo Kasai
“Vacuolar sequential exocytosis of large dense-core vesicles in adrenal medulla”
EMBO J., vol.25, pp.673-82 (2006) doi: 10.1038/sj.emboj.7600983

Hiroyasu Hatakeyama, Takuya Kishimoto, Tomomi Nemoto, Haruo Kasai and *Noriko Takahashi
“Rapid glucose sensing by protein kinase A for insulin exocytosis in mouse pancreatic islets”
J. Physiol., vol.570, pp.271-282 (2006) DOI: 10.1113/jphysiol.2005.096560

2005

Ting-ting Liu, Takuya Kishimoto, Hiroyasu Hatakeyam, Tomomi Nemoto, Noriko Takahashi, *Haruo Kasai
“Exocytosis and endocytosis of small vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis”
J. Physiol., vol. 568, pp. 917-29 (2005) DOI: 10.1113/jphysiol.2005.094011

Takuya Kishimoto, Ting-ting Liu, Hiroyasu Hatakeyama, Tomomi Nemoto, Noriko Takahashi, *Haruo Kasai
“Sequential compound exocytosis of large dense-core vesicles in PC12 cells studied with TEPIQ (two-photon extracellular polar-tracer imaging-based quantification) analysis”
J. Physiol., vol.568, pp.905-15 (2005) DOI: 10.1113/jphysiol.2005.094003

Takuya Kishimoto , Ting-ting Liu , Tomomi Nemoto & Noriko Takahashi
“A new quantitative (TEPIQ) analysis for diameters of exocytic vesicles and its application to pancreatic islets”
J. Physiol., vol.568, pp.891-903 (2005) DOI: 10.1113/jphysiol.2005.093047

Akihiro Oshima, Tatsuya Kojima, Kenji Dejima, Yasuo Hisa, Haruo Kasai, and *Tomomi Nemoto
“Two-photon microscopic analysis of acetylcholine-induced mucus secretion in guinea pig nasal glands”
Cell Calcium, vol.37, pp.349-357 (2005) DOI: 10.1016/j.ceca.2004.12.003

Yasuyuki Hayakawa, Tomomi Nemoto, Masamitsu Iino, *Haruo Kasai
“Rapid Ca2+-dependent increase in oxygen consumption by mitochondria in single mammalian central neurons”
Cell Calcium, vol.37, pp.359-370 (2005) DOI: 10.1016/j.ceca.2004.11.005

2004

Tomomi Nemoto, Tatsuya Kojima, Akihiro Oshima, Haruhiko Bito and Haruo Kasai
“Stabilization of exocytosis by dynamic F-actin coating of zymogen granules in pancreatic acini”
Biol. Chem., vol.279, pp.37544-37550 (2004)

2003

Takahashi, N., Kishimoto, T., Nemoto, T., Kasai, H.
“Two-photon imaging of insulin exocytosis in the pancreatic islets”
J. Pharmacolog. Sci., vol.91, pp.32P-32P (Suppl.), (2003)

2002

Noriko Takahashi, Takuya Kishimoto, Tomomi Nemoto, Takashi Kadowaki, *Haruo Kasai
“Fusion pore dynamics and insulin granule exocytosis in the pancreatic islet”
Science, vol.297, pp.1349-52 (2002) DOI:10.1126/science.1073806

Mitsuhiko Noda, Shigeo Yamashita, Noriko Takahashi, Kazuhiro Eto, Linming Shen, Kazuo Izumi, Samira Daniel, Yoshiharu Tsubamoto, Tomomi Nemoto, Masamitsu Iino, Haruo Kasai, Geoffrey W.G. Sharp, and *Takashi Kadowaki
“Switch to anaerobic glucose metabolism with NADH accumulation in the ß-cell model of mitochondrial diabetes: Characteristics of ßHC9 cells deficient in mitochondrial DNA transcription”
J. Biol. Chem. DOI: 10.1074/jbc.M207690200

Tomomi Nemoto, Ryoichi Kimura, Akira Tachikawa, Akiko Miwa, Haruo Okado, Yasushi Miyashita, Masamitsu Iino, Takashi Kadowaki and Haruo Kasai
“Two-photon excitation imaging of pancreatic islet with various fluorescent probes.”
Diabetesvol.S1, pp.25-28 (2002) DOI: 10.2337/diabetes.51.2007.S25

2001

Tomomi Nemoto, Ryoichi Kimura, Koichi Ito, Akira Tachikawa, Yasushi Miyashita, Masamitsu Iino, and *Haruo Kasai
“Sequential Replenishment Mechanism of Exocytosis in Pancreatic Acini”
Nature Cell Biol.vol.3, pp.253-258 (2001) DOI: 10.1038/35060042

Masanori Matsuzaki, Graham C. R. Ellis-Davies, Tomomi Nemoto, Yasushi Miyashita, Masamitsu Iino, *Haruo Kasai
“Dendritic spine geometry is ciritical for functional AMPA receptor expression in hippocampal CA1 pyramidal neurons”
Nature Neurosci.vol.4, No.11, pp.1086-1092 (2001) DOI: 10.1038/nn736

Noriko Takahashi, Tomomi Nemoto , Haruo Kasai
“Two-photon excitation imaging of insulin exocytosis from intact pancreatic islet of Langerhans”
DIABETOLOGIAvol.44, p.231 (2001)

1998

Go Uchida, Yuuki Mizukami, Tomomi Nemoto, Yoshimi Tsuchiya
“Sliding Motion of Magnetizable Beads Coated with Chara Motor Protein in a Magnetic Field”
J. Phys. Soc. Jpn., vol. 67, pp. 345-350 (1998)

1995

Tomomi Nemoto, Go Uchida, Atsuko Takamatsu, Yoshimi Tsuchiya
“Stochastic Behaviors of Organelle Motion in Nitella Internodal Cells”
Biochem. Biophys. Res. Comm., vol.214, No.3, pp.1102-1107 (1995) DOI: 10.1006/bbrc.1995.2399

Go Uchida, Tomomi Nemoto, Yoshimi Tsuchiya
“Characteristics of Sliding Motions of Small Organelles in a Nitella Internodal Cell
J. Phys. Soc. Jpn., vol.64, No.12, pp.4959-4963 (1995) DOI: 10.1143/JPSJ.64.4959