2018/11/30 NIPS/Thermal Biology Training Course Application Closed!
A FAOPS2019 satellite - NIPS/Thermal Biology Training Course
- National Institute for Physiological Sciences (NIPS) [located at Okazaki] and Thermal Biology Group supported by JSPS will hold a training course
on basic techniques in physiological research on April 1st-5th 2019 at
NIPS. The course covers the travel expense from Kobe to NIPS and from NIPS
to the airport of the return flight as well as the accommodation fee for
five nights at NIPS.
September 1st -
October 30, 2018 November 30, 2018.
Please apply by October 31 for a greater chance for your success, but we
will still accept application until Novermber 30.
- Candidates need participation registration for 9th FAOPS Congress 2019.
The schedule has been revised.
National Institute for Physiological Sciences (NIPS) is an inter-university
research institute for research and education on human physiology, which
investigates the mechanisms of human body function. NIPS also carries out
joint studies with domestic and foreign scientists, and provides education
and training for graduate students and young scientists. Followed by FAOPS2019
at Kobe, NIPS will hold a training course on basic techniques in physiological
research for the participants of FAOPS2019 in collaboration with Scientific
Research on Innovative Areas ‘Thermal Biology’ from April 1st to 5th, 2019.
We welcome applications from young researchers such as graduate students
and postdoctoral fellows with keen interest and enthusiasm in physiological
- Patch-Clamp and Thermal Imaging (Tominaga lab)
We will teach the fundamental methods to analyze functions of ion channels
in vitro using patch-clamp and imaging techniques. We will also demonstrate
plasmid transfection and data processing. The first part of the training
is electrophysiological analyses of temperature-sensitive TRP channels
(thermo TRPs) that are transiently expressed in tissue culture cells. Thermo
TRPs are unique set of receptors in primary afferent neurons that are activated
pharmacologically and physically including temperature increase/decrease.
Trainees will learn the theoretical basis of patch-clamp technique and
practical methods to stimulate and record the activities of the thermo
TRPs upon ligand/temperature stimulations. The second part of the training
is a simultaneous recording of activities of the thermo TRPs and temperature
changes using imaging techniques. Recent advances in fluorescent probes
allow us to measure heterogeneity and changes of temperature in subcellular
region. Trainees will learn how to measure temperature fluctuations and
intracellular Ca2+ increase upon local heating using a confocal microscope.
We will accept 7 young researchers (graduate students and Postdocs) who
are interested in or going to use these techniques in future. The applicants
do not need special skills/backgrounds of electrophysiology and molecular
biology, and novices from every field are encouraged to submit.
Link to Tominaga Lab.
- Application has been closed.
Patch-clamp recording from neurons in acute brain slices (Yoshimura lab)
The whole-cell patch-clamp technique makes it possible to analyze synapses
and local neural circuits in a brain slice preparation in which the fundamental
architecture of the circuits is mostly maintained. In this training course,
we will teach trainees how to prepare acute slices from mouse visual cortex
and record membrane potentials and excitatory/inhibitory postsynaptic currents
from pyramidal neurons on the slice using the whole-cell patch-clamp technique.
In addition, we will provide instructions on how to perform a functional
analysis of neural circuits employing a combination of laser scanning photostimulation
using caged-glutamate and whole-cell recording. We will accept 2-3 young
researchers for participation in this training course. We welcome graduate
students and Postdocs who are interested in the electrophysiological analysis
of functional synapses and neural circuits in the mammalian brain. The
applicants do not need special skills or backgrounds in electrophysiology.
Link to Yoshimura Lab.
- Application has been closed.
In vitro/vivo approaches for evaluating cardiovascular functions (Nishida
In our training program, we will walk you through some of the fundamental
techniques to investigate molecular and physiological basis of cardiovascular
adaptations and pathologies. These include both in vitro and in vivo approaches
Isolation of cardiomyocytes from rat neonatal
Isolation of cardiomyocytes from mouse adult heart
Ca2+ imaging using isolated cardiomyocytes
Echocardiography (non-invasive measurement of
ail-cuff (non-invasive measurement of blood pressure)
Laser speckle flowgraphy (non-invasive measurement
of peripheral blood flow)
We will accept 2-3 dedicated future scientists (graduate students and Postdocs)
who are interested in exploring cardiovascular systems by using our techniques.
Although specialized knowledge or skills are not necessarily required,
experience with standard molecular biology techniques is preferred.
Link to Nishida Lab.
- Biochemical analysis of in vivo protein complexes (Fukata lab)
Determining the composition of native protein complexes is essential for
understanding the physiological and pathological processes of living organisms.
We will introduce a series of experiments, which allow high recovery and
specific isolation of the protein complexes in vivo. In this course, trainees
will practice affinity purification of protein complexes from the brain
lysate, silver staining and in-gel digestion of purified protein complexes
and mass spectrometry-based protein identification. We will accept 2-3
young researchers (graduate students and postdocs).
Link to Fukata Lab.
- Immunofluorescence staining of frozen tissue sections (Furuse lab)
To determine the cellular or tissue localization of a protein of interest
provides crucial information to understand its function. Immunofluorescence
staining is commonly used for this purpose. It can also be used as a qualitative
measure of protein expression. In this course, we will train beginners
to acquire a series of a protocol for immunofluorescence staining of frozen
tissue sections, including preparation of frozen sections, immunostaining,
image acquisition with a fluorescence microscope and data presentation.
We will accept 2 young researchers (graduate students and postdocs).
Link to Furuse Lab.