The structures of six states of a rotary sodium ion pump are revealed using cryo-electron microscopy.

Raymond N. Burton Smith, Chihong Song, Hiroshi Ueno, Takeshi Murata, Ryota Iino, Kazuyoshi Murata* (2023) Six states of Enterococcus hirae V-type ATPase reveals non-uniform rotor rotation during turnover. Comm Bio 6 755. WEB

The detailed particle structure of Tanaivirus was revealed using cryo-electron microscopy.

Kenta Okamoto*, Chihong Song, Han Wang, Miako Sakaguchi, Christina Chalkiadaki, Naoyuki MIyazaki, Takeshi Nabeshima, Kouichi Morita*, Shingo Inoue, and Kazuyoshi Murata (2023) Structure and its transformation of elliptical nege-like virus Tanay virus. J Gen Vir 104(6),001863. WEB

New factor of mitochondrial fission in mitophagy was discovered and the process was visualized by 3D-SEM.

Tomoyuki Fukuda#, Kentaro Furukawa#, Tatsuro Maruyama#, Shun-Ichi Yamashita, Daisuke Noshiro, Chihong Song, Yuta Ogasawara, Kentaro Okuyama, Jahangir Md. Alam, Manabu Hayatsu, Tetsu Saigusa, Keiichi Inoue, Kazuho Ikeda, Akira Takai, Lin Chen, Vikramjit Lahiri, Yasushi Okada, Shinsuke Shibata, Kazuyoshi Murata, Daniel J. Klionsky, Nobuo N. Noda*, Tomotake Kanki* (2023) The mitochondrial intermembrane space protein mitofissin drives mitochondrial fission required for mitophagy. Mol Cell 83(12),2045-2058. WEB

The unique structure of acient type PSI in Ostreococcal was revealed using cryo-electron microscopy.

Asako Ishii, Jianyu Shan, Xin Sheng, Eunchul Kim, Akimasa Watanabe, Makio Yokono, Chiyo Noda, Chihong Song, Kazuyoshi Murata, Zhenfeng Liu, Jun Minagawa (2023) The photosystem I supercomplex from a primordial green alga Ostreococcus tauri harbors three light-harvesting complex trimers. eLife 84488. WEB

Structure of a giantvirus, tokyovirus, was revealed at 7.7Å resolution by high-voltage cryo-EM single particle analysis.

Chihara A, Burton-Smith RN, Kajimura N, Mitsuoka K, Okamoto K, Song C, Murata K (2022) A novel capsid protein network allows the characteristic inner membrane structure of Marseilleviridae giant viruses. Sci Rep 12(1):21428. WEB

Hydrated structures of nano-composite microgels produced with different components were revealed by cryo-electron tomography.

Nishizawa Y, Watanabe T, Noguchi T, Takizawa M, Song C, Murata K, Minato H, Suzuki D (2022) Durable gelfoams stabilized by compressible nanocomposite microgels. Chem Commun (Camb) 58, 12927-12930. WEB

The structure of the sapovirus shell (capsid), one of the causative viruses of mass diarrhea, was clarified for the first time in the world at the atomic level.

Miyazaki N, Song C, Oka T, Miki M, Murakami K, Iwasaki K, Katayama K, Murata K* (2022) Atomic structure of human sapovirus capsid by single particle cryo-electron microscopy revealed a unique capsid protein conformation in caliciviruses. J Virol 96(9), e0029822. WEB

The neural circuit (connectomics) of the brain region where the light information entering from the compound eye of the swallowtail butterfly is initially processed has been clarified.

Matsushita A, Stewart F, Ilić M, Chen PJ, Wakita D, Miyazaki N, Murata K, Kinoshita M, Belusic G, Arikawa K (2022) Connectome of the lamina reveals the circuit for early color processing in the visual pathway of a butterfly. Cur biology 32(10):2291.e3, 00506-1. WEB

Medusavirus, a giant virus, shows a unique maturation process in host amoeba.

Watanabe R, Song C, Kayama Y, Takemura M, Murata K* (2022) Particle morphology of Medusavirus inside and outside cells reveals new maturation process of giant virus. J Viol 96(7), e0185321. WEB

A neutralized VHH antibody for SARS-CoV2 was developed and its binding model was revealed by cryo-EM.

Haga K, Takai-Todaka R, Matsumura Y, Song C, Takano T, Tojo T, Nagami A, Ishida Y, Masaki H, Tsuchiya M, Ebisudani T, Sugimoto S, Sato T, Yasuda H, Fukunaga K, Sawada A, Nemoto N, Murata K, Morimoto T, Katayama K. (2021) Nasal delivery of single-domain antibody improves symptoms of SARS-CoV-2 infection in an animal model. PLoS Pathog 17(10):e1009542. doi: 10.1371/journal.ppat.1009542. WEB

CAHS protein in targigrade was revealed to form a fiber in desiccated condition.

Yagi-Utsumi M, Aoki K, Watanabe H, Song C, Nishimura S, Satoh T, Yanaka S, Ganser C, Tanaka S, Schnapka V, Goh EW, Furutani Y, Murata K, Uchihashi T, Arakawa K, Kato K (2021) Desiccation-induced fibrous condensation of CAHS protein from an anhydrobiotic tardigrade. Sci Rep 11(1), 21328. doi: 10.1038/s41598-021-00724-6. WEB

Helicobacter pylori bacteriophage exhibits a capsid structure to avoid digestion in stomach.

Kamiya R, Uchiyama J, Matsuzaki S, Murata K, Iwasaki K, Miyazaki N (2021) Acid-stable capsid structure of Helicobacter pylori bacteriophage KHP30 by single-particle cryo-electron microscopy. Structure 30(2):300-312.e3. doi: 10.1016/j.str.2021.09.001 WEB

Cotonvirus, a giant virus descovered in Japan uses Golgi apparatus for its virion factory.

Takahashi H, Fukaya S, Song C, Murata K, Takemura M (2021) Cotonvirus japonicus using Golgi apparatus of host cells for its virion factory phylogenetically links tailed tupanvirus and icosahedral mimivirus.J Virol 95(18),e0091921. doi: 10.1128/JVI.00919-21. WEB

A phytoplankton isodated in north see was found to produces oil.

Harada N, Hirose Y, Song C, Kurita H, Sato M, Onodera J, Murata K, Itoh F (2021) A novel characteristic of a phytoplankton as a potential source of straight-chain alkanes.Sci Rep 11(1), 14190. WEB

The state transition structure of PS-I was revealed in Chlamydomonas.

Pan X , Tokutsu R, Li A, Takizawa K, Song C, Murata K, Yamasaki T, Liu Z, Minagawa J, Li M (2021) Structural basis of LhcbM5-mediated state transitions in green algae.Nature plant 7(8), 1119-1131. WEB

The double ring consisting of the proteasome α7 subunit has large fluctuations, which provide hints for the formation of the proteasome complex.

Song C, Satoh T, Sekiguchi T, Kato K*, Murata K* (2021) Structural fluctuations of the human proteasome α7 homo-tetradecamer double ring imply the proteasomal α-ring assembly mechanism. Int J Mol Sci 22(9), 4519. WEB

The high-end TITAN Krios G4  (TFS) having a 300kV cold-field emission gun and a pos- column energy filter (Selectris X) is used for single particle anaysis, electron tomography, and micrED.

Gun: 300kV C-FEG
Energy filter: Post column Selectris X
Camera: Falcon4
Software: EPU

The electron microscope JEM2200FS (JEOL) having a 200kV Thermal Schottky and an in-column energy filter, is used for grid screening and training for cryo-EM.

Gun: 200kV Thermal Schottky FEG
Specimen holder: Gatan 622 cryo-specimen holder
Energy filter: In-column Omega-type
Camera: DE-20

Cryo-FIB-SEM, Aquilos2 (TFS) is used for producing a thin cryo-section of biological samples. An additional fluoescence microscope unit (iFLM) makes it easy to find the target area .

SEM: Thermal Schottky FEG
FIB: Ga Liquid metal ion source
CLEM: iFLM