f

Yuko Fukata, M.D. and Ph.D.

yfukata

Brief History

1988-1994
Kobe University School of Medicine, Kobe, Japan.

1994-1995
Resident of Pediatrics, Kobe University Hospital, Kobe, Japan. (Prof. Hajime Nakamura)

1997-2000
Doctoral Research Fellow of the Japan Society for the Promotion of Science for Young Scientists
Nara Institute of Science and Technology, Nara, Japan. (Prof. Kozo Kaibuchi)

2000
PhD, Nara Institute of Science and Technology, Nara, Japan. (Prof. Kozo Kaibuchi)

2000-2003
Postdoctoral Fellow of the Japan Society for the Promotion of Science for Young Scientists
Nagoya University Graduate School of Medicine, Nagoya, Japan. (Prof. Kozo Kaibuchi)

2003-2005
Postdoctoral Fellow, University of California at San Francisco, CA. (Prof. David S Bredt)

2003-2005
Long-Term Fellow of Human Frontier Science Program

2005-
Postdoctoral Fellow, National Institute for Longevity Sciences, Aichi, Japan (Chief, Masaki Fukata)

2007.6-
Researcher, Division of Membrane Physiology, Department of Cell Physiology,
National Institute for Physiological Sciences (NIPS) (Prof. Masaki Fukata)

2007.12-
Associate Professor, Division of Membrane Physiology, Department of Cell Physiology,
National Institute for Physiological Sciences (NIPS)

Achievement (-2007)

Yamamoto, N., Fukata, Y., Fukata, M., and Yanagisawa, K. GM1-ganglioside-induced Abeta assembly on synaptic membranes of cultured neurons. Biochim Biophys Acta. 1768, 1128-1137 (2007).

Tomita, S., Shenoy, A., Fukata, Y., Nicoll, R.A., and Bredt, D.S. Stargazin interacts functionally with the AMPA receptor glutamate-binding module. Neuropharmacology. 52, 87-91 (2007).

Fukata, Y., Adesnik, H., Iwanaga, T., Bredt, D.S., Nicoll, R.A., and Fukata, M. Epilepsy-related ligand/receptor complex LGI1 and ADAM22 regulate synaptic transmission. Science. 313, 1792-1795 (2006).

Fukata, Y., Iwanaga, T., Fukata, M. Systematic screening for palmitoyl transferase activity of the DHHC protein family in mammalian cells. Methods. 40, 177-182 (2006).

Fang, C., Deng, L., Keller, C.A., Fukata, M., Fukata, Y., Chen, G., and Luscher, B. GODZ-mediated palmitoylation of GABA(A) receptors is required for normal assembly and function of GABAergic inhibitory synapses. J. Neurosci. 26, 12758-12768 (2006).

Fernandez-Hernando, C., Fukata, M., Bernatchez, P.N., Fukata, Y., Lin, M.I., Bredt, D.S., and Sessa, W.C. Identification of Golgi-localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase. J. Cell Biol. 174, 369-377 (2006).

Fukata, Y., Bredt, DS., and Fukata, M. Protein palmitoylation by DHHC protein family. The CRC Press The Dynamic Synapse: Molecular Methods in Ionotropic Receptor Biology. 81-88 (2006).

Fukata, Y., Tzingounis, AV., Trinidad, JC., Fukata, M., Burlingame, AL., Nicoll, RA., and Bredt, DS. Molecular constituents of neuronal AMPA receptor. J Cell Biol. 169, 399-404 (2005).

Arimura, N., Celine, M., Kawano, Y., Yoshimura, T., Kawabata, S., Hattori, A., Fukata, Y., Amano, M., Goshima, Y., Inagaki, M., Morone, N., Usukura, J., and Kaibuchi, K. Phosphorylation by Rho-kinase regulates CRMP-2 activity in growth cones. Mol. Cell Biol.　25, 9973-9984 (2005).

Kimura, T., Arimura, N., Fukata, Y., Watanabe, H., Iwamatsu, A., and Kaibuchi, K. Tubulin and CRMP-2 complex is transported via kinesin-1. J. Neurochem. 93, 1371-1382 (2005).

Fukata, M., Fukata, Y.,Adesnik, H, Nicoll, RA., and Bredt, DS. Identification of PSD-95 palmitoylating enzymes. Neuron. 44, 987-996 (2004).

Nishimura, T., Kato, K., Yamaguchi, T., Fukata, Y., Ohno, S., and Kaibuchi, K. Role of the PAR-3-KIF3 complex in the establishment of neuronal polarity. Nature Cell Biol. 5, 328-334 (2004).

Celine, M., Arimura, N., Fukata, Y., and Kaibuchi, K. PIP3 is involved in neuronal polarization and axon formation. J. Neurochem. 89, 109-118 (2004).

Noritake, J., Fukata, M., Sato, K., Nakagawa, M., Watanabe, T., Wang, S., Izumi, N., Wang, S., Fukata, Y., and Kaibuchi, K. Positive role of IQGAP1, an effecter of Rac1, in actin-meshwork formation at sites of cell-cell contact. Mol. Biol Cell. 15, 1065-1076 (2004).

Arimura, N., Menager, C., Fukata, Y., Kaibuchi, K. Role of CRMP-2 in neuronal polarity. J Neurobiol. 58, 34-47 (2004).

Amano M, Kaneko T, Maeda A, Nakayama M, Ito M,Yamauchi T, Goto H, Fukata Y., Oshiro N, Shinohara A, Iwamatsu A, and Kaibuchi K. Identification of Tau and MAP2 as novel substrates of Rho-kinase and myosin phosphatase. J. Neurochem. 87, 780-790 (2003).

Nishimura, T., Fukata, Y., Katsuhiro, K., Yamaguchi, T., Matsuura, Y., Kamiguchi, H. and Kaibuchi, K. CRMP-2 regulates polarized Numb-mediated endocytosis for axon growth, Nature Cell Biol. 5, 819-826 (2003).

Maeda, A., Amano M., Fukata, Y., and Kaibuchi K. Translocation of Na+,K+ATPase is induced by Rho small GTPase in renal epithelial cells, Biochem. Biophys. Res. Commun. 297, 1231-1237 (2002).

Fukata, Y., Itoh, T.J., Kimura, T., Menager, C., Nishimura, T., Shiromizu, T., Watanabe, H., Inagaki, N., Iwamatsu, A., Hotani, H., and Kaibuchi, K. CRMP-2 binds to tubulin heterodimers to promote microtubule assembly, Nature Cell Biol. 4, 583-591 (2002).

Fukata, Y., Kimura, T., and Kaibuchi, K. Neuronal Polarity: Axon specification in hippocampal neurons, Neuroscience Res. 43, 305-315, (2002).

Fukata, Y., Amano, M., and Kaibuchi, K. Rho/Rho-kinase pathway in smooth muscle contraction and cytoskeletal reorganization of non-muscle cells, Trend. Pharmacol. Sci. 22, 32-39, (2001).

Kandabashi, T., Shimokawa, H., Miyata, K., Kunihiro, I., Kawano, Y., Fukata, Y., Higo, T., Egashira, K., Takahashi, S., Kaibuchi, K. and Takeshita, A. Inhibition of myosin phosphatase by upregulated rho-kinase plays a key role for coronary artery spasm in a porcine model with interleukin-1beta, Circulation 101, 1319-1323, (2000).

Nakamura, N., Oshiro, N., Fukata, Y., Amano, M., Fukata, M., Kuroda, S., Matsuura, Y., Leung, T., Lim, L. and Kaibuchi, K. Phosphorylation of ERM proteins at filopodia induced by Cdc42, Genes Cells 5, 571-581 (2000).

Amano, M., Fukata, Y., Shimokawa, H. and Kaibuchi, K. Purification and in Vitro Activity of Rho-Associated Kinase, Methods Enzymol. 325, 149-155 (2000).

Amano, M., Fukata, Y. and Kaibuchi, K. Regulation and Functions of Rho-Associated Kinase, Exp. Cell Res. 261, 44-51 (2000).

Izawa, T., Fukata, Y., Kimura, T., Iwamatsu, A., Dohi, K. and Kaibuchi, K. Elongation Factor-1alpha Is a Novel Substrate of Rho-Associated Kinase, Biochem. Biophys. Res. Commun. 278, 72-78 (2000).

Fukata, Y., Oshiro, N. and Kaibuchi, K. Activation of moesin and adducin by Rho-kinase downstream of Rho, Biophys. Chem. 82, 139-147 (1999).

Fukata, Y., Oshiro, N., Kinoshita, N., Kawano, Y., Matsuoka, Y., Bennett, V., Matsuura, Y. and Kaibuchi, K. Phosphorylation of adducin by Rho-kinase plays a crucial role in cell motility, J. Cell Biol. 145, 347-361 (1999).

Kawano, Y., Fukata, Y., Oshiro, N., Amano, M., Nakamura, T., Ito, M., Matsumura, F., Inagaki, M. and Kaibuchi, K. Phosphorylation of myosin-binding subunit (MBS) of myosin phosphatase by Rho-kinase in vivo, J. Cell Biol. 147, 1023-1038 (1999).

Kimura, K., Fukata, Y., Matsuoka, Y., Bennett, V., Matsuura, Y., Okawa, K., Iwamatsu, A., and Kaibuchi, K. Regulation of the association of adducin with actin filaments by Rho-kinase and myosin phosphatase, J. Biol. Chem. 273, 5542-5548, (1998).

Oshiro, N., Fukata, Y., and Kaibuchi, K. Phosphorylation of Moesin by Rho-associated Kinase (Rho-kinase) Plays a Crucial Role in the Formation of Microvilli-like Structures, J. Biol. Chem. 273, 34663-34666 (1998).

Amano, M., Chihara, K., Nakamura, N., Fukata, Y., Yano, T., Shibata, M., Ikebe, M., and Kaibuchi, K. Myosin II activation promotes neurite retraction during the action of Rho and Rho-kinase, Genes Cell 3, 177-188 (1998).

Amano, M., Fukata, Y., and Kaibuchi, K. Regulation of Cytoskeleton and Cell Adhesions by the Small GTPase Rho and Its Targets, Trend Cardio. Med. 8, 162-168 (1998).

Fukata, Y., Kimura, K., Oshiro, N., Saya, H., Matsuura, Y. and Kaibuchi, K. Association of the myosin-binding subunit of myosin phosphatase and moesin: dual regulation of moesin phosphorylation by Rho-kinase and myosin phosphatase, J. Cell Biol. 141, 409-418 (1998).

Amano, M., Chihara, K., Kimura, K., Fukata, Y., Nakamura, N., Matsuura, Y., and Kaibuchi, K. Formation of actin stress fibers and focal adhesions by Rho-kinase, Science 275, 1308-1311 (1997).

Kimura, K., Ito, M., Amano, M., Chihara, K., Fukata, Y., Nakafuku, M., Yamamori, B., Feng, J., Nakano, T., Okawa, K., Iwamatsu, A., and Kaibuchi, K. Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase), Science 273, 245-248 (1996).

Amano, M., Ito, M., Kimura, K., Fukata, Y., Chihara, K., Nakano, T., Matsuura, Y., and Kaibuchi, K. Phosphorylation and activation of myosin by Rho-associated kinase (Rho-kinase), J. Biol. Chem. 271, 20246-20249 (1996).