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Division of Cardiocirculatory Signaling

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Elucidation of biological functions using multilevel techniques to evaluate cardiovascular functions and its clinical application

Our sanguiferous function is mainly controled by muscular organs composed of striated
muscles (heart and skeletal muscles) and smooth muscle (blood vessels). Our group aims to elucidate the molecular mechanisms underlying transition of the muscles from adaptation to maladaptation against environmental stress (mainly hemodynamic load) multi-level techniques to evaluate cardiovascular functions (in vivo and in vitro), and work toward practical application (e.g., drug discovery and fostering). We also investigate the mechanism of muscle repair and regeneration, and aim to develop a novel therapeutic strategy for refractory diseases. In addition, we address the inclusive research to elucidate the mechanism underlying maintenance and transfiguration of cardiocirculatory homeostasis via multi-organ interactions by combining non-invasive measuring methodologies of motor functions and those cardiovascular functions.
Our laboratory has various techniques and equipments to drive the above researches.

1. Non-invasive measurements of muscular functions
Echo-cardiography (mouse and rat), Laser Doppler flowmetry (mouse), Measuring devices of motor activity (mouse), Tail-cuff (mouse and rat), blood pressure telemetry (mouse)
2. Invasive measurements of cardiovascular functions
Langendorff perfusion system (mouse and rat), Mouse millar catheter (for P-V loop
measurement)
3. Isolation of primary-cultured cells and experiments
mechanical stretching machine, Ca2+ imaging, FRET imaging, Confocal laser microscopy, Patch-clamp recording, Plate reader (BRET assay, post-translational modification analyses)

Figure.  Measuring systems for cardiovascualr functions and summary of our research using these systems

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Typical paper information

*T. Numaga-Tomita et al., Sci. Rep. 6, 39383 (2016)
*N. Kitajima et al., Sci. Rep. 6, 37001 (2016)
*A. Nishimura et al., Sci. Signal. 9, ra7. (2016)
*S. Mangmool et al., Mol. Endocrinol. 30, 118 (2016)
*T. Numaga-Tomita et al., Biochem J. 473, 201 (2016)
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