Research

Section of Multilayer Physiology

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In vivo analysis of neuronal and metabolic activity, and behavioral patterns in mice and rats

This section analyzes the in vivo neuronal and metabolic activity, and behavioral patterns in mice and rats which have been modified by their related genes and by exposure to various environmental conditions.
This section performs the following examinations:
  •  Single unit recording from motor related brain regions in an awake state (Figure 1).
  • Behavioral analysis for the evaluation of learning and memory, and emotion: Morris water maze, Barnes maze, Fear    conditioning, Passive avoidance, Rota-rod, Open field, Elevated plus maze, Light-dark transition, Forced swimming, Y maze, 3-chamber social interaction, etc. (Figure 2).
  • Behavioral analysis under group housing using automated, remote monitoring system, IntelliCage.
  • Regional neural activity detected as intrinsic signals with taking the advantage of light fluorescent dynamics of flavin or hemoglobin.
  • Measurement of non-invasive echo-graphic imaging of tissue structure-function relationships (liver, kidney and blood vessels), 4-dimensional changes in cardiac functions, and capillary blood flow (brain and umbilical cord) using anesthetized mice.


図1_250321_173346.jpg

Figure 1. To elucidate pathophysiology of L-DOPA-induced dyskinesia, neuronal activity of basal ganglia neurons in response to electrical stimulation of the motor cortex was analyzed.


図2_改変_250321_173358.jpgFigure 2. We perform a number of behavioral analyses in mice to explore physiological functions of specific genes and molecules.


 

Selected publications

*Polyakova et al. J Neurosci 44: e1911222024 (2024)
*Hasegawa et al. Nat Commun 13: 2233 (2022)
*Chiken et al. Cereb Cortex 31: 5363-5380 (2021)
*Dwi Wahyu I et al. J Neurosci 41: 2668-2683 (2021)
*Watanabe et al. Nat Commun 11: 3253 (2020)