Behavioral and physiological rhythms in most living organisms are regulated by the molecular clock system. Alterations in molecular clocks works are associated with various diseases such as cancer, metabolic syndrome, and hypertension. Currently, treatment for such diseases remains unsatisfactory, resulting in unmet medical needs. Hepatocellular carcinoma (HCC) is one such disease with unmet medical needs. Chronic inflammatory and HCC are induced by infection of hepatitis B and C viruses. Interferon (IFN) therapy reduce HCC. However, existing therapy methods are insufficient for correcting chronic hepatitis and HCC.
 Therefore, we investigated the influence of molecular clock function on the development of chronic inflammatory and HCC. The 24-h expression of clock genes and Ccrf were altered in liver at 14 weeks after treatment of diethylnitrosamine (DEN). The microarray analysis in Ccrf knockdown hepatic cells showed the alteration of pathway in carcinoma and cell cycle. Addition, function of Ccrf protein was regulated the inflammatory signal. The present time, high throughput chemical screening has been performed for discovery of Ccrf inhibitor. Molecular clock function is very important as a new target in the treatment of Chronic inflammatory and HCC.