Division of Homeostatic Development


  Remodeling of Neuronal Circuits in Development and Recovery,
−In vivo Imaging and Electrophysiological Study-

Remodeling of neuronal circuits takes place during development, learning, and recovery after brain damage. The main goal of our research is to understand the regulation of neural circuits remodeling. In detail, we are focusing on glial contribution to the function of neuronal circuits. Glia is a key factor to regulate neural circuits through their physiological function. We are trying to determine their contribution to the neural circuits in physiological and pathological conditions by visualizing fine structure, controlling activity, and recording specific synaptic transmissions in living animals using multi-photon microscopy. We are also focusing on experience-dependent remodeling in sensory neural circuits during development. We use behavioral analysis, in vivo imaging, and in vitro electrophysiology to clarify the correlation between the development of behavioral patterns and synaptic plasticity as its basis.

Innovative technology development across fields

The progress of technology has brought about a breakthrough in life science. We contributed to novel technology development through collaborative research across fields. We recently revealed neural activity-dependent pH changes in the living brain with single-cell level resolution using a CMOS image sensor which we had newly developed.


Fig1. CMOS-based ion image sensor revealed neuronal activity dependent pH changes in the living brain



Fig2.  Pain circuit reorganization with activated astrocytes as a therapeutic approach



Selected publications

*参考文献① Cortical astrocytes rewire somatosensory cortical circuits for peripheral neuropathic pain. Kim SK, Hayashi H, Ishikawa T, Shibata K, Shigetomi E, Shinozaki Y, Inada H, Roh SE, Kim SJ, Lee G, Bae H, Moorhouse AJ, Mikoshiba K, Fukazawa Y, Koizumi S, Nabekura J. J Clin Invest. 2016 May 2;126(5):1983-97.
*参考文献② CMOS-based bio-image sensor spatially resolves neural activity-dependent proton dynamics in the living brain. Horiuchi H, Agetsuma M, Ishida J, Nakamura Y, Cheung DL, Nanasaki S, Kimura Y, Iwata T, Takahashi K, Sawada K, Nabekura J. Nat Commun. 2020 Feb 5;11(1):712.